Thermo Fisher Scientific Human Insulin Recombinant Protein

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

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Miscellaneous PubMed (Misc)

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View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

Human Insulin is a two chain, non-glycosylated polypeptide containing 51 amino acids.

Molecular weight

5.8 kDa

Class

Recombinant

Type

Protein

Activity

Fully biologically active as compared to WHO reference standard at 28 U/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

purified

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

This is a recombinant protein suitable for use as a control in ELISA and Western blot applications.

Lyophilized Insulin is stable at room temperature for 3 weeks. Reconstitute in sterile 0.005N HCl to a concentration of no more than 1mg/mL. Store lyophilized product at -20ºC. Upon reconstitution, store at 4ºC for 2-7 days or aliquot and store at -20ºC. For long term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.