Thermo Fisher Scientific Human alpha-1 PDX Recombinant Protein

Thermo Fisher Scientific Human alpha-1 PDX Recombinant Protein

Applications and Test Information

Product Specifications

Product Specific Information

RP-070 was obtained from E. coli transformed with the alpha1-PDX gene.
It can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0–20 µM alpha1-PDX for 1 hour. For in vitro assays, 0.1–2.0 µM alpha1-PDX should be used.
Purity: >80% (most lots are 80–95%)

Notes:

• Freezing may damage the protein.
• Once rehydrated, retains optimal reactivity for 2–4 days at 4°C.
• Longer storage possible in serum-rich medium.
• Suitable for biochemical, intracellular trafficking, and protein kinetic assays.
• Molecular weight: approximately 46.7 kDa.

Target Information

Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM).
It was engineered as a variant of alpha1-antitrypsin containing a minimal furin consensus sequence (RXXR).
Cellular expression of alpha1-PDX effectively blocks furin-dependent processing of protein precursors.
Extracellular application also inhibits processing of furin substrates.
Alpha1-PDX has been shown to inhibit processing of biologically significant proteins including viral envelope glycoproteins, bacterial toxins, receptors, and growth factors.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.