Thermo Fisher Scientific Human alpha-1 PDX Recombinant Protein

Applications

Tested Dilution

Publications

Neutralization (Neu)

-

View 1 publication 1 publication

Control (Ctrl)

Assay-dependent

-

Inhibition Assays (IA)

Cell studies, 8.0-20 µM

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Human

Expression System

E. coli

Molecular weight

46.7 kDa

Class

Recombinant

Type

Protein

Purity

>80%

Activity

For furin inhibition assays, cells should be treated with 8.0 - 20 µM human alpha-1 PDX recombinant protein for 1 hour. For in vitro assays, 0.1 - 2.0 µM human alpha-1 PDX recombinant protein should be used.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

Product Specific Information

RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20 µM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 µM alpha1-PDX should be used. Purity: >80% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays.

Molecular weight is approximately 46.7 kDa.

Target Information

Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.