Thermo Fisher Scientific IL-5 Monoclonal Antibody (5A10)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5A10

Immunogen

Recombinant human IL-5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223574

Product Specific Information

M551 targets IL-5 in ELISA, and WB applications and shows reactivity with Human samples.

The M551 immunogen is recombinant human IL-5.

M551 detects IL-5 which has a predicted molecular weight of approximately 13 kDa.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

The M551 IL5 antibody (clone 5A10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M550B (biotinylated conjugate of clone 39D10). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M551 (clone 5A10) and biotinylated antibody M550B (clone 39D10) have successfully been used in combination with recombinant IL5 protein SIL5 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.