Thermo Fisher Scientific IL-2 Monoclonal Antibody (B-G5), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B-G5

Immunogen

Recombinant human IL-2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223575

Product Specific Information

M600B targets IL-2 in ELISA, and WB applications and shows reactivity with Human samples.

The M600B immunogen is recombinant human IL-2.

M600B detects IL-2 which has a predicted molecular weight of approximately 15 kDa.

The M600B IL2 antibody (biotinylated conjugate of clone BG5) has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody P600. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M600B (clone BG5) and antibody P600 have successfully been used in combination with recombinant IL2 protein SIL2 in ELISA applications.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.