Thermo Fisher Scientific CXCL13 Monoclonal Antibody (143614)
Mouse CXCL13 단백질을 인식하는 Rat IgG2a 단일클론 항체. ELISA 및 중화 실험에 적합하며, 높은 특이성과 낮은 엔도톡신 수준을 보유. 인간 CXCL13 등과 교차 반응 없음. 단백질 A/G로 정제된 동결건조 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 2–8 µg/mL |
| Neutralization | 12–30 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 143614 |
| Immunogen | E. coli-derived recombinant mouse CXCL13 (Ile22–Ala109) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610213 |
Product Specific Information
- In ELISAs, this antibody does not cross-react with recombinant human CXCL13, rmCXCL11/I-TAC, rmCXCL9/MIG, or rmCXCL12/SDF-1α.
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).
Target Information
CXCL13 (also known as BCA-1 and Angie) is a small chemokine strongly expressed in secondary lymphoid organs. It promotes migration of B lymphocytes (B-1 and B-2) to follicles of the spleen, lymph nodes, and Peyer’s patches.
It is produced by macrophages, with high expression in large peritoneal macrophages, Kupffer cells, and lung macrophages (CD11c+ and CD11c-).
The receptor for CXCL13 is CXCR5 (BLR-1), expressed on B cells, Tfh cells, and some memory T cell subsets.
CXCL13 plays an important role in chronic lymphocytic leukemia (CLL) and serves as a plasma biomarker for germinal center activity and broadly neutralizing antibody generation against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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