Thermo Fisher Scientific Notch1 Monoclonal Antibody (OTI3E12), TrueMAB
Human Notch1 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. 고순도 Affinity chromatography로 정제된 Lyophilized 형태. 연구용으로만 사용하며, 재구성 및 결합 실험에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E12 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 2300–2556 of human NOTCH1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The Notch gene family encodes transmembrane proteins with multiple cysteine-rich EGF-like repeats in the extracellular region. Four Notch genes (Notch1, Notch2, Notch3, Notch4/Int-3) are known in mammals and are involved in differentiation of the nervous system and other structures.
EGF-like proteins Delta and Serrate act as ligands for Notch1. Mature Notch proteins form heterodimeric receptors composed of an extracellular subunit (NEC) and a transmembrane subunit (Ntm).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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