Thermo Fisher Scientific JNK1 Monoclonal Antibody (OTI4E3), TrueMAB
JNK1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간 시료 반응성, 정제된 Lyophilized 형태. PBS buffer에 8% 트레할로스 포함, -20°C 보관. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–384 of human JNK1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK8 (JNK1) is activated by the presence of misfolded proteins in the endoplasmic reticulum. It is a crucial mediator of insulin resistance and obesity, belonging to the MAPK superfamily of stress-activated protein kinases.
MAPKs are serine-threonine protein kinases activated by various extracellular stimuli, mediating signal transduction from the cell surface to the nucleus.
JNK is involved in cellular processes such as proliferation, differentiation, transcription regulation, and development. JNK pathways are activated by stress and inflammatory signals.
JNK exists as ten isoforms due to differential mRNA splicing, with JNK1 and JNK2 being predominant forms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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