Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Super Bright 780, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Super Bright 780, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 506
• FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Request custom conjugation

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b, associated with integrin beta2 (CD18), is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for ICAM family members (CD54, CD102, CD50) and iC3b, essential for cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted, tested at 5 µL (0.5 µg)/test
• Excitation: 405 nm (violet laser)
• Emission: 780 nm
• Recommended Filter: 780/60 bandpass

Super Bright 780 Dye Characteristics

• Tandem dye excited by violet laser (405 nm), emits at 780 nm
• Sensitive to photo-induced oxidation — protect from light
• Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes
• Compensation values may differ between beads and cells; set compensation using stained cells for accuracy

Fixation Guidance

• Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C (dark).
• Clone-specific performance after fixation should be determined empirically.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that forms a heterodimer with integrin beta-2 (CD18), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.

• Functions as receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X
• Commonly used as a microglial marker in nervous tissue
• Expression varies with macrophage type and tissue
• Associated diseases include systemic lupus erythematosus and ITGAM-related susceptibility to SLE

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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