Thermo Fisher Scientific MMP25 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 321-539 of human MMP25 (NP_0719131) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MMP25,uniProtId:Q9NPA2-1,ncbiNodeId:9606,antigenRange:321-539,antigenLength:562,antigenImageFileName:PA5-90790_MMP25_Q9NPA2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90790_MMP25_Q9NPA2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.62 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806238

Product Specific Information

Immunogen sequence: FDAIANIRGE TFFFKGPWFW RLQPSGQLVS PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQA

Positive Samples: A-549, HT-29, Mouse lung, Mouse liver, Rat liver; Cellular Location: Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, extracellular matrix, extracellular space

Target Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.