Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• View 1 publication

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 or Leu9.
CD7, a 40 kDa receptor, is part of the immunoglobulin gene superfamily. The N-terminal region (aa1–107) is homologous to Ig kappa light chains, while the C-terminal extracellular domain is proline-rich, forming a stalk for the Ig domain.

CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, natural killer cells, a subset of normal B cells, and malignant B cells.
Cross-linking of CD7 enhances T cell and NK cell activation, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3'-kinase, inducing D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells expressing CD7 has lymphopoietic potential, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells. CD7(+) phenotype distinguishes primitive lymphoid progenitors from pluripotent stem cells.

CD7 may cooperate with CD28 during Treg function, as CD28/CD7-deficient mice show reduced Treg numbers and suppressive activity.

Applications Reported

• Reported for use in flow cytometric analysis.

Applications Tested

• Pre-titrated and tested by flow cytometry on normal human peripheral blood cells.
• Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10^5–10^8 cells/test).

Handling and Storage

• Light Sensitivity: Protect from light (photo-induced oxidation).
• Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily.
It is expressed on most immature and mature T lymphocytes, T cell leukemias, natural killer cells, and subsets of normal and malignant B cells.
CD7 signaling modulates T cell and NK cell activity and is associated with phosphoinositol 3'-kinase activation.
Expression of CD7 serves as an important marker in leukemia diagnostics.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지