Thermo Fisher Scientific TCP-1 theta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 284 and 527 of Human TCP1 theta if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCP-1 theta,uniProtId:P50990-1,ncbiNodeId:9606,antigenRange:284-527,antigenLength:548,antigenImageFileName:PA5-30403_TCP-1_theta_P50990-1_Rabbit.svg,antigenImageFileNamePDP:PA5-30403_TCP-1_theta_P50990-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.99 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547877

Product Specific Information

Recommended positive controls: 293T, A431, Jurkat, Raji.

Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (84%), Xenopus laevis (87%), Chicken (95%), Rhesus Monkey (99%), Chimpanzee (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TCP-1 theta is theta subunit of the CCT (chaperonin containing TCP1 complex) chaperonin molecule which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of the newly synthesized proteins. Molecular chaperones assist in the protein folding upon ATP hydrolysis and TCP-1 theta may play a role in the assembly of the BBsome, a complex that is involved in ciliogenesis regulating transport vesicles to the cilia. Further, TCP-1 theta is also involved in the folding of actin and tubulin. The intact CCT complex is composed of eight polypeptides in a double ring structure. CCT is important within cells in the folding of the VHL tumor suppressor protein.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.