Thermo Fisher Scientific NCKAP1 Polyclonal Antibody
Human 및 Mouse 반응성을 가진 Rabbit Polyclonal 항체로, Western Blot 및 Immunohistochemistry에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. U87-MG 세포를 양성 대조로 권장하며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 2–338 of Human NCKAP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.36 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547880 |
Product Specific Information
- Recommended positive control: U87-MG
- Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (92%), Xenopus laevis (94%), Rhesus Monkey (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene interacts with Nck and may be involved in downstream signaling of Nck. It shares similarity with the mouse Dhm1 and yeast dhp1 genes, which play roles in homologous recombination and RNA metabolism such as RNA synthesis and trafficking. Complementation studies indicate that Dhm1 has similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted, though their full-length nature is unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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