Thermo Fisher Scientific Ubiquitin Recombinant Rabbit Monoclonal Antibody (FK2), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

All, Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

FK2

Immunogen

Ubiquitin conjugated lysozyme

Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931540

Product Specific Information

A 1:1,000 dilution of MA5-45086 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.