Thermo Fisher Scientific HSP70 Monoclonal Antibody (C92F3A-5), PerCP
HSP70 단백질을 검출하기 위한 Thermo Fisher Scientific의 PerCP 결합 단클론 항체. 다양한 종에 반응하며 WB, IHC, ICC, Flow Cytometry 등 다중 응용 가능. 높은 특이성과 민감도로 HSC70과 교차 반응 없음. 연구용으로 적합.
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Thermo Fisher Scientific HSP70 Monoclonal Antibody (C92F3A-5), PerCP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:1,000 |
| ELISA | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, C. elegans, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C92F3A-5 |
| Immunogen | Human HSP70 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931546 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45092 was sufficient for detection of HSP70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 70 kDa. Does not cross-react with HSC70 (HSP73).
Target Information
HSP70 is a heat shock protein expressed in response to various biological stresses, including high temperatures. The HSP70 family includes highly conserved proteins induced by stress in all examined organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70: Constitutively expressed, involved in clathrin-coated vesicle uncoating
- GRP78 (BiP): 78 kDa, localized in the endoplasmic reticulum
- p75 (HSP75): Found in mitochondria
HSP70 is encoded by an intronless gene and, together with other heat shock proteins, stabilizes existing proteins and assists in folding newly translated proteins. It is also involved in the ubiquitin-proteasome pathway through interaction with AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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