Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (N27F3-4), APC
HSP70/HSC70 단백질 검출용 APC 결합 단일클론 항체로, 다양한 종에 반응하며 WB, IHC, ICC, Flow 등 여러 응용에 적합. 고순도 Protein G 정제, 1 mg/mL 농도, 4°C 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (N27F3-4), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, C. elegans, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Non-human primate, Sheep, Plant, Pig, Rabbit, Rat, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N27F3-4 |
| Immunogen | Recombinant HSP70/HSC70 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931547 |
Available Formats
- FITC ([MA5-45094])
- PerCP ([MA5-45095])
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45093 was sufficient for detection of HSP70/HSC70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 72 kDa (HSP) and approximately 73 kDa (HSC).
Target Information
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a protein-coding gene associated with diseases such as Chagas Disease and Babesiosis. Related pathways include regulation of activated PAK-2p34 by proteasome-mediated degradation and cellular responses to stimuli.
An important paralog of this gene is HSPH1.
HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is also a protein-coding gene associated with auditory system disease and Borna disease. Related pathways include processing of capped intron-containing pre-mRNA and selective autophagy.
Gene Ontology (GO) annotations include RNA binding and ubiquitin protein ligase binding.
An important paralog of this gene is HSPA2.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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