Thermo Fisher Scientific NSG1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

NSG1, also known as Neuron-specific protein family member 1, contains an N-terminal nuclear localization motif and a central hydrophobic segment. NSG1 localizes to the nucleus in mitotic neuronal cell cultures and to the cytoplasm in postmitotic neurons. Mouse and human NSG1 share 97% sequence identity, differing by only five amino acid residues. The protein is expressed in all regions of the rat brain and preferentially in adult hamster brain.

GenScript Rabbit Anti-NSG1 Polyclonal Antibody is developed in rabbit using purified His-tagged human NSG1 (Swiss Prot: P42857) full length recombinant protein.

Target Information

NEEP21 (neuron-enriched endosomal 21 kDa protein), also known as brain neuron cytoplasmic protein 1, NSG1 (neuron-specific proteins family member 1), P21, or D4S234E, is a single-pass type II membrane protein belonging to the NSG family. It is highly expressed during neuronal maturation but downregulated in adult tissues.

NEEP21 predominantly localizes to Rab4-positive early endosomes in the somatodendritic neuronal compartment and is essential for proper receptor sorting and recycling in neurons. It associates with GRIP1 and GluR-2, mediating surface expression of GluR-2. When this interaction is interrupted, GluR-2 accumulates in early endosomes, leading to altered synaptic current properties.

Additionally, NEEP21 forms a complex with the SNARE protein Syntaxin 13 (also known as Syntaxin 12) and participates in recycling of transferrin receptors (TFRs) and NTR2 (Neurotensin receptor 2).

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.