Thermo Fisher Scientific Phospho-Tau (Ser396) Polyclonal Antibody

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Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Tau is a microtubule-associated phosphoprotein (MAP) localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, ranging from 352 to 441 amino acids. The tau variants differ with the presence of either three or four repeat regions near the carboxy-terminal and one or two inserts near the amino-terminal.

Tau is hyperphosphorylated by ERK, GSK-3β, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Ser396, which is one of the major abnormal phosphorylation sites of tau. Phosphorylation at these sites reduces tau’s ability to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein found in paired helical filaments (PHFs), forming the neurofibrillary tangles characteristic of Alzheimer’s disease (AD) and other CNS disorders.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both structures.

Axonal polarity is influenced by TAU/MAPT localization in the neuronal cell body domain defined by the centrosome. Short isoforms provide cytoskeletal plasticity, while longer isoforms contribute to stabilization.

In its hyperphosphorylated form, Tau is the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease brains. Hyperphosphorylation impairs Tau’s microtubule-binding function, leading to destabilization and neuronal degeneration.

Numerous kinases phosphorylate Tau, including GSK-3β, PKA, CDK5, and casein kinase II. Hyperphosphorylated Tau is observed in neurofibrillary lesions of Alzheimer’s disease, Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.