Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 532, eBioscience
마우스 CD4 분자에 특이적인 GK1.5 단클론 항체로, Alexa Fluor 532로 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 534/553 nm 파장에서 여기/방출되며, 4°C 암소 보관이 권장됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 90 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 532, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2802358 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Yellow series
- eFluor 450, 506, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7
- Super Bright series
- NovaFluor series
- Request custom conjugation
Product Specific Information
Description:
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subset of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is critical for T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤0.5 µg per test (100 µL final volume). Optimal titration is advised for each assay. Typical cell count range: 10⁵–10⁸ cells/test.
Fluorochrome Information:
Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. It is not compatible with the Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass. Confirm instrument compatibility before use.
Excitation: 532 nm
Emission: 561 nm
Laser: Green Laser
Target Information
CD4 antigen is a co-receptor for MHC class II molecules and HIV, expressed mainly on T helper cells, and also on monocytes, macrophages, and dendritic cells. It is found in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut. CD4 participates in T-cell activation via association with the T-cell receptor complex and Lck kinase. It may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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