Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 532, eBioscience
인간 CD4 항원을 인식하는 RPA-T4 단클론 항체로, Alexa Fluor 532 형광체가 결합되어 있습니다. Flow cytometry 및 IHC 분석에 적합하며, HIV 수용체 차단 특성을 가집니다. 534/553 nm 파장에서 여기/방출되며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | 1:25 | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 19 publications |
| Neutralization (Neu) | - | View 2 publications |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 532, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T4 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation/Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802361 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585 / 690
- Pacific Orange
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed on most thymocytes, a subpopulation of mature T cells (T-helper cells), and at low levels on monocytes. CD4 functions as a receptor for HIV, and RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a distinct epitope from the OKT4 antibody, and they do not cross-block each other.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells. Use 5 µL (0.125 µg) per test in a final 100 µL volume. Cell count range: 10⁵–10⁸ cells/test.
Fluorophore Information:
Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. Not compatible with the Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass.
Excitation: 532 nm
Emission: 561 nm
Laser: Green Laser
Target Information
CD4 is a co-receptor involved in the recognition of MHC class II molecules and serves as an HIV receptor. It is expressed on T helper cells, monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions. CD4 assists in T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck, and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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