Thermo Fisher Scientific SPTBN1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Spectrin Non-Erythroid Beta 1 (SPTBN1). Validated for WB, IHC(P), and IP applications. Unconjugated liquid form with 0.20 mg/mL concentration. Recommended storage at 4°C and shelf life of 1 year.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2100 and 2150 of Human Spectrin Non-Erythroid Beta 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
Spectrin (Sp), the most abundant erythrocyte membrane skeleton protein, maintains the biconcave shape and flexibility of red blood cells. It consists of a 280 kDa alpha subunit and a 246 kDa beta subunit forming a 100 nm rod-like structure. Spectrin in non-erythroid tissues may contain homologous alpha and beta subunits (fodrin). The nomenclature designates erythrocyte subunits as alpha-1 and beta-1, and fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms are labeled epsilon-1, epsilon-2, etc. (e.g., beta-1 epsilon-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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