Thermo Fisher Scientific Phospho-TrkB (Tyr816) Polyclonal Antibody
Phospho-TrkB (Tyr816) 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot 및 IHC에 적합하며, TrkB 인산화 부위 특이적 검출에 유용합니다. 동결건조 형태로 제공되며, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 10–50 µg/mL |
| Immunohistochemistry (IHC) | 10–50 µg/mL |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 730–821 (including phospho Y816 site) of rat TrkB conjugated to a blue carrier protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in human and mouse.
- Specificity: TrkB.
Target Information
NTRK2 (TRKB) belongs to the neurotrophic factor family of related polypeptides central to the development and maintenance of the mammalian nervous system.
NTRK2 is the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses.
TrkB is involved in the development and maintenance of the nervous system and is a tyrosine kinase gene highly related to TrkA. TrkB expression is confined to tissues within the central and peripheral nervous systems.
BDNF and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine residues of TrkB gp145, with BDNF eliciting a stronger response. TrkB, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway, leading to cell differentiation.
Mutations in the TrkB gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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