Thermo Fisher Scientific CD52 Monoclonal Antibody (CF1D12), PerCP-eFluor 710, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

CF1D12

Immunogen

Human CD52

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

View additional formats

• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762460

Product Specific Information

Description: This CF1D12 monoclonal antibody reacts with human CD52, a cell surface glycoprotein expressed in the immune and reproductive systems. CF1D12 is thought to recognize the carbohydrate region of CD52, not the protein core of the molecule. Tissue-specific modifications of the glycan structure may alter epitope recognition in certain applications. For example, this CF1D12 clone has been reported to recognize human sperm glycoforms of CD52 by western blot, but not by flow cytometry. The immunogen used to generate this antibody was human CD52.

Applications Reported: This CF1D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CF1D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Target Information

CD52, also known as CAMPATH-1 antigen, is a glycoprotein that is expressed on the surface of various immune cells, including T and B lymphocytes, natural killer cells, and monocytes. It is involved in the regulation of immune responses and is being studied as a potential therapeutic target in various diseases, including multiple sclerosis, chronic lymphocytic leukemia, and autoimmune disorders. CD52 is the target of alemtuzumab, a monoclonal antibody that is used to treat these conditions by depleting immune cells that express CD52.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.