Thermo Fisher Scientific CD58 (LFA-3) Monoclonal Antibody (TS2/9), PerCP-eFluor 710, eBioscience
인체 CD58(LFA-3)을 인식하는 TS2/9 단일클론 항체로, PerCP-eFluor 710 형광 표지. Flow Cytometry에 최적화된 사용량(5 µL/test)으로 T세포 활성화 및 세포 부착 연구에 활용. 빛에 민감하며 4°C 암소 보관 권장.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TS2/9 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762461 |
Additional Formats
Product Specific Information
The TS2/9 antibody recognizes human CD58 (Lymphocyte Associated Antigen-3, LFA-3). CD58 exists as both a transmembrane and a GPI-linked extracellular protein, and both forms can be expressed concurrently. Soluble CD58 has been observed in serum, urine, and synovial fluid of rheumatoid arthritis patients. CD58 is expressed on non-hematopoietic and most hematopoietic cells.
Binding to its ligand CD2 on T cells initiates signaling pathways leading to cytokine release. CD58 plays roles in cell adhesion, T cell activation, cytokine production, and cytotoxicity. The TS2/9 clone can induce signaling upon crosslinking, and the epitope is located in domain 1 of the immunoglobulin region.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry using human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per 100 µL test volume (10⁵–10⁸ cells/test).
Fluorochrome Information:
PerCP-eFluor™ 710 emits at 710 nm and is excited at 488 nm (blue laser). Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored up to 3 days at 4°C (in dark) using:
- IC Fixation Buffer (Product #00-8222)
- 1-step Fix/Lyse Solution (Product #00-5333)
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Target Information
This gene encodes a member of the immunoglobulin superfamily. The protein acts as a ligand for CD2 on T lymphocytes, mediating adhesion and activation. It is localized to the plasma membrane, and alternative splicing variants are known.
[RefSeq, Jan 2009]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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