Thermo Fisher Scientific Calpain S1/S2 Monoclonal Antibody (28F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-1 µg/mL

-

Immunoprecipitation (IP)

1-2 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

28F3

Immunogen

Full length protein (Human). Antibody specifically recognizes small subunit. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpain S1/S2,uniProtId:P04632-1,ncbiNodeId:9606,antigenRange:1-268,antigenLength:268,antigenImageFileName:MA1-34966_Calpain_S1S2_P04632-1_House_mouse.svg,antigenImageFileNamePDP:MA1-34966_Calpain_S1S2_P04632-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.5, with 50% glycerol

Contains

3mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2069488

Product Specific Information

This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). It recognizes both native and denatured protein in ELISA, Western Blot and Immunoprecipitation.

Target Information

The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and m-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, m-calpain is active at micromolar (mM) concentrations of Ca++. In addition to the ubiquitously expressed m- and m- calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.