Thermo Fisher Scientific Calpain S1/S2 Monoclonal Antibody (28F3)
Calpain S1/S2 단백질의 작은 서브유닛(28~30 kDa)을 특이적으로 인식하는 마우스 모노클로날 항체. Western blot, ELISA, Immunoprecipitation 등 다양한 응용에 적합. 단백질 G로 정제된 액상 형태로, 장기 보관 시 -20°C에서 안정적.
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Thermo Fisher Scientific Calpain S1/S2 Monoclonal Antibody (28F3)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | - |
| Flow Cytometry (Flow) | – | 1 publication |
| ELISA | 0.5–1 µg/mL | - |
| Immunoprecipitation (IP) | 1–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 28F3 |
| Immunogen | Full length protein (Human). Antibody specifically recognizes small subunit. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.5, with 50% glycerol |
| Contains | 3 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2069488 |
Product Specific Information
This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). It recognizes both native and denatured protein in ELISA, Western Blot, and Immunoprecipitation.
Target Information
Calpains are calcium-dependent cysteine proteases widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) consist of an 80 kD large subunit and a 30 kD small subunit. The small subunit is common to both enzymes, while the large catalytic subunits differ and have distinct calcium requirements. μ-calpain is active at micromolar Ca²⁺ concentrations, whereas m-calpain requires millimolar levels. Various tissue-specific calpains have been identified, and their physiological roles continue to evolve.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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