Thermo Fisher Scientific GST Polyclonal Antibody, Biotin
GST 태그 단백질 검출용 비오틴 결합 폴리클로날 항체. Western blot, IHC, ELISA, IP에 적합. Rabbit IgG 기반으로 높은 특이성과 재현성 제공. 친화 크로마토그래피로 정제되어 고순도 보장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:80,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length GST isolated from Schistosoma japonicum |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | 4°C (short term), -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Immunoelectrophoresis result:
Single precipitin arc observed against anti-biotin, anti-rabbit serum, as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum).
Cross-reactivity with GST from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein found in both eukaryotes and prokaryotes, catalyzing various reactions. It is widely used to create GST gene fusion systems, allowing purification of GST-fusion proteins via their high affinity for glutathione. These fusion proteins are valuable tools for studying protein–protein interactions. The GST gene fusion system is incorporated into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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