Thermo Fisher Scientific GFP Polyclonal Antibody, Biotin
GFP 단백질을 특이적으로 인식하는 Biotin 결합 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용 가능. 높은 특이성과 안정성 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:5,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Green Fluorescent Protein (GFP) produced by immunizing GFP-containing fusion protein corresponding to full-length amino acid sequence (246aa) from Aequorea victoria |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below; avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
- Immunoelectrophoresis assay showed a single precipitin arc against anti-Rabbit Serum, anti-Biotin, and purified or partially purified GFP (Aequorea victoria).
- No reaction observed against Human, Mouse, and Rat Serum Proteins.
Target Information
The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. GFP technology has provided significant insights into cellular physiology.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment, absorbing blue light (395 nm) and emitting green light (508 nm) without additional cofactors.
Its fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, protein–protein interaction studies (yeast two-hybrid system), and FRET-based distance measurements.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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