Thermo Fisher Scientific GFP Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot and freeze at -20°C or below; avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
• Immunoelectrophoresis assay showed a single precipitin arc against anti-Rabbit Serum, anti-Biotin, and purified or partially purified GFP (Aequorea victoria).
• No reaction observed against Human, Mouse, and Rat Serum Proteins.

Target Information

The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. GFP technology has provided significant insights into cellular physiology.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment, absorbing blue light (395 nm) and emitting green light (508 nm) without additional cofactors.
Its fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, protein–protein interaction studies (yeast two-hybrid system), and FRET-based distance measurements.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.