Thermo Fisher Scientific PARP (Poly (ADP-ribose) polymerase) Monoclonal Antibody (10H)
PARP 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로 인간 시료에 반응. Western blot 및 IHC(P) 실험에 적합. 핵 내 PARP 검출에 활용되며 apoptosis 연구에 유용. 단백질 A로 정제된 액상 항체로 안정적 저장 가능.
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Thermo Fisher Scientific PARP (Poly (ADP-ribose) polymerase) Monoclonal Antibody (10H)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H |
| Immunogen | Recombinant protein of human PARP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Testis tissues
- Cellular location: Nucleus
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form, NAD) as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) mediated by the caspase CPP32 protein.
Cleavage occurs between Adp216 and Gly217, a conserved site across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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