Thermo Fisher Scientific Human TGF-beta 3 Recombinant Protein
인간 유래 TGF-beta 3 재조합 단백질로, 세포 신호전달 및 면역 조절 연구에 활용됩니다. E. coli 발현 시스템에서 생산되며, >98% 순도와 낮은 엔도톡신 수준을 보장합니다. 다양한 어세이(WB, IHC, ELISA)에 적용 가능하며 높은 생물학적 활성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human TGF-beta 3 recombinant protein is a homodimer containing two 112 amino acid subunits |
| Molecular Weight | 25.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 0.05 ng/mL (≥ 12.5×10⁶ IU/mg); determined by a toxicity assay using the WHO Standard 98/608 for direct comparison |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | 0.12% acetic acid with 20% ethanol |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
- Purity: >98% by SDS-PAGE and HPLC
- Endotoxin Concentration: <0.1 ng/µg
- Protein Length: 112 aa (2 identical subunits)
- Biological Activity: ED50 ≤ 0.05 ng/mL
- Molecular Weight: 17.5 kDa
Target Information
Transforming growth factor beta 3 (TGF-beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF-beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer.
As with all members of the family, TGF-beta 3 is highly conserved across species, with mouse and human TGF-beta 3 demonstrating 100% sequence homology and cross-species activity. Members of this family can be expressed by most cell types and exert pleiotropic effects, including suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation.
Additionally, TGF-beta cytokines promote CD4⁺CD25⁺ T-cell expansion, indicating an important role in protection against autoimmunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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