Thermo Fisher Scientific Heat Stable FGF-10 Recombinant Protein

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Thermostable variant of human FGF-10 (190 amino acids) with proprietary amino acid substitutions

Class

Recombinant

Type

Protein

Purity

> 95% by SDS-PAGE

Endotoxin concentration

0.25 EU/µg

Activity

ED50 ≤ 1.0 ng/mL; measured in a cell proliferation assay using 4MBr-5 cells

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

750mM NaCl, 20mM Na2PO4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Native FGF-10 is highly unstable under normal cell culture conditions. Gibco Human Heat Stable FGF-10 Recombinant Protein has been engineered for greater stability in cell culture, exhibiting =80% activity at 37°C for at least 24 hours. Gibco Human Heat Stable FGF-10 Recombinant Protein is bioactive longer than native FGF-10 in standard cell culture conditions allowing researchers to possibly use less protein to maintain the same growth rates, improving the efficiency of their cell culture applications.
Gibco Human Heat Stable FGF-10 Recombinant Protein has >90% sequence homology to native FGF-10 making it a suitable replacement for native FGF-10 in most cell culture applications.
Learn more at the Gibco Heat Stable Recombinant Protein webpage

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 1.0 mg/mL. Alternatively, reconstitute at lower concentrations in carrier-containing medium. Do not vortex. Apportion the reconstituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in medium, or buffered solution containing carrier protein, such as PBS with 0.1% BSA. For the 5 µg unit size, reconstitute directly in carrier-containing medium. For other unit sizes, reconstitute to 1.0 mg/mL as follows:

• 50 µg, reconstitute in 50 µL
• 100 µg, reconstitute in 100 µL
• 500 µg, reconstitute in 500 µL

Storage: Store the lyophilized protein at -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C (not in a frost-free freezer) for up to 12 months. Avoid repeated freeze-thaw cycles.

Target Information

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.