Thermo Fisher Scientific Phospho-eNOS (Ser1177) Polyclonal Antibody
Phospho-eNOS (Ser1177) 항체로 인산화된 eNOS 단백질을 특이적으로 검출. Western blot, IHC, ICC/IF에 사용 가능. Rabbit polyclonal IgG 형식으로 1 mg/mL 농도. PBS/glycerol buffer에 보관하며 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NOS3 (Accession P29474), corresponding to amino acid residues around phosphorylated Ser1177. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816331 |
Product Specific Information
This antibody detects endogenous levels of eNOS only when phosphorylated at Serine 1177.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that mediates intercellular signaling, including vasorelaxation, neurotransmission, and cytotoxicity. NO production is catalyzed by nitric oxide synthase (NOS) family enzymes, which convert L-arginine to L-citrulline and NO. There are three NOS isoforms:
- nNOS (type I) – neuronal NOS
- iNOS (type II) – inducible NOS
- eNOS (type III) – endothelial NOS
All NOS isoforms contain binding domains for calmodulin, NADPH, FAD, and FMN.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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