Thermo Fisher Scientific GST Monoclonal Antibody (3D4), DyLight 488
DyLight 488 형광 표지를 가진 GST 단클론 항체(3D4)로, Western blot, ICC/IF, ELISA에 적합. BALB/c 마우스에서 유래한 IgG1, kappa 형 항체이며 GST 태그 검출에 특화. 단백질 A로 정제되어 높은 특이성과 재현성을 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D4 |
| Immunogen | BALB/c mice were immunized with GST from full length Schistosoma japonicum protein. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology. |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- DyLight 405
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- Request custom conjugation available
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and purified and partially purified Glutathione-S-Transferase (GST).
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein present in eukaryotes and prokaryotes, where it catalyzes a variety of reactions. Glutathione S-transferase is used to create the GST gene fusion system. This GST-fusion protein can be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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