Thermo Fisher Scientific PDPK1 Polyclonal Antibody
PDPK1 단백질의 내인성 수준을 검출하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, ICC/IF에 적합합니다. 높은 특이성과 재현성을 제공하며, 연구용으로 PDPK1 관련 신호전달 및 대사 연구에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PDPK1 (Accession O15530), corresponding to amino acid residues D216–W266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818430 |
Product Specific Information
Antibody detects endogenous levels of total PDPK1.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. It activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to activation of glycogen synthase and stimulation of glycogen synthesis. Due to its role in insulin-induced glycogen synthesis and PKC activation, PDPK1 is an important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-99497_PDPK1_O15530-1_Rabbit.svg, PA5-99497_PDPK1_O15530-1_Rabbit_PDP.jpeg)
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