Thermo Fisher Scientific IKK alpha/beta Polyclonal Antibody
IKK alpha/beta 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 시료에 반응하며, Affinity chromatography로 정제됨. PBS/glycerol buffer에 보관하며 -20°C에서 안정적으로 저장 가능.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHUK (Accession O15111), corresponding to amino acid residues V155–W205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818429 |
Product Specific Information
This antibody detects endogenous levels of total IKK alpha/beta.
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) genes located on chromosomes 10 and 8 respectively in humans.
Upon stimulation by agents that activate the NF-κB pathway such as TNF-alpha, the two subunits of the IKK complex (IKK alpha and IKK beta) together with the regulator NEMO (IKK gamma) phosphorylate IκB proteins. This leads to ubiquitin-mediated degradation of IκB and phosphorylation of NF-κB, resulting in its translocation to the nucleus.
Phosphorylation at serine residues 176 and 180 in the activation loop induces a conformational change required for kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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