Thermo Fisher Scientific BECN1 Monoclonal Antibody (OTI4A10), TrueMAB
BECN1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow Cytometry에 적합. 인간 시료 반응성. 동결건조 형태로 제공되며 PBS buffer에 보존. 오토파지 연구 및 세포 내 단백질 분석에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A10 |
| Immunogen | Full length human recombinant protein of human BECN1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Beclin 1 plays a central role in autophagy as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.
Different PI3K complex forms are believed to participate in multiple membrane trafficking pathways:
- PI3KC3-C1 is involved in the initiation of autophagosomes.
- PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis.
Beclin 1 also contributes to antiviral host defense mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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