Thermo Fisher Scientific BCAR1 Monoclonal Antibody (OTI1A9), TrueMAB
BCAR1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. HEK293T 세포에서 생산된 full length human BCAR1를 면역원으로 사용. Lyophilized 형태로 제공되며, PBS 및 trehalose buffer에 보존. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A9 |
| Immunogen | Full length human recombinant protein of human BCAR1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The p130Cas (Crk-associated substrate) is a common target of phosphorylation signals mediated by v-Crk and v-Src oncoproteins.
It contains an SH3 domain followed by multiple YXXP motifs and a proline-rich region.
p130Cas is involved in various biological processes such as:
- Cell adhesion and migration
- Growth factor stimulation
- Cytokine receptor engagement
- Bacterial infection
Physiological functions include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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