Thermo Fisher Scientific Phospho-DARPP-32 (Thr34) Polyclonal Antibody
Phospho-DARPP-32 (Thr34) 항체로서 Western blot에 최적화되어 있으며 Mouse 및 Rat에서 반응합니다. 합성 인산화 펩타이드 면역원으로 제작된 Rabbit Polyclonal IgG 항체입니다. 신경전달 조절 연구에 적합하며 -20°C에서 보관합니다.
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Applications
- Western Blot (WB)
Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat PPP1R1B conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_560608 |
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, human, mouse, and non-human primate based on 100% sequence homology.
In Western blot, this antibody detects an ~32 kDa protein representing Phospho-Thr34 DARPP-32 protein.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when phosphorylated on Thr34. Conversely, phosphorylation on Thr75 turns DARPP-32 into a PKA inhibitor.
Phosphorylation of DARPP-32 plays a critical role in dopaminergic neurotransmission regulation and is implicated in the effects of alcohol, caffeine, and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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