Thermo Fisher Scientific Transcription Factor IIB Monoclonal Antibody (IIB8)
Transcription Factor IIB 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 ELISA에 사용 가능. 인간 및 마우스 시료에 반응하며, Protein A로 정제된 액상 제품. 보존제가 없으며 -20°C에서 보관.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IIB8 |
| Immunogen | Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. This process regulates the concentrations of key regulatory proteins by limiting their lifespans. Polyubiquitination is a common feature of this modification. Steps include activation of ubiquitin, ATP-dependent formation of a thioester bond with E1 enzyme, transfer to E2 enzyme, and covalent linkage to target proteins directly or via E3 ligase. Deubiquitination enzymes can reverse this process. Ubiquitination is involved in various cellular processes such as cell cycle, growth, differentiation, apoptosis, signal transduction, DNA repair, transcription regulation, and immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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