Thermo Fisher Scientific Histone H3 Recombinant Rabbit Monoclonal Antibody (24HC2 LC12)
Recombinant rabbit monoclonal antibody specific to Histone H3. Validated for WB, ICC/IF, ELISA, and ChIP. Offers high specificity, lot-to-lot consistency, and animal-origin-free formulation. Suitable for chromatin and transcription research.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg |
| ELISA | Assay-Dependent |
| ChIP assay | 1.5 µg / 1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 24HC2 LC12 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2848509 |
Product Specific Information
This antibody is predicted to react with bovine, non-human primate, rat, and ovine based on sequence homology.
On a non-reducing gel, intact IgG appears as a ~150 kDa band, and upon reduction, generates a ~25 kDa light chain and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in chromatin of all eukaryotic cells. Together with four core histones, it forms the nucleosome structure. Histones are key regulators of transcription, DNA repair, replication, and chromosomal stability.
The N-terminal tail of Histone H3 can undergo multiple post-translational modifications, such as methylation or acetylation of lysine and arginine residues, and phosphorylation of serine or threonine. These modifications influence chromatin structure and transcription factor binding, impacting diverse cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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