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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 700, eBioscience

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인간 IFN-γ를 인식하는 4S.B3 단클론 항체로, Alexa Fluor 700으로 표지되어 Flow Cytometry에 최적화됨. 인트라셀룰러 염색에 사용 가능하며, 633 nm 레이저로 여기되어 723 nm에서 방출. 면역조절 연구 및 세포 활성화 분석에 적합.

카탈로그번호: 56-7319-42
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 56-7319-42 IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 700, eBioscience 100 tests pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 700, eBioscience™

Applications and Tested Dilution

Application	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 70 publications
ELISA	-	View 1 publication

Product Specifications

Specification	Details
Species Reactivity	Human
Published Species	Mouse
Host / Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	4S.B3
Conjugate	Alexa Fluor™ 700
Excitation/Emission Max	696 / 719 nm
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2574509

Available Formats

Unconjugated
Alexa Fluor 488
Alexa Fluor 660
APC
APC-eFluor 780
Biotin
Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
Brilliant Violet 650
eFluor 450 / 506 / 660
FITC
PE / PE-Cyanine7 / PE-eFluor 610
PerCP-Cyanine5.5
Request custom conjugation

Product Specific Information

The 4S.B3 monoclonal antibody reacts with human interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells. IFN-γ exhibits antiviral and antiparasitic activity and inhibits proliferation of normal and transformed cells in synergy with other cytokines such as TNF-α.

This antibody is validated for intracellular staining and flow cytometric analysis using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and BestProtocols®: Protocol A for cytoplasmic proteins. Recommended usage is 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Alexa Fluor® 700 emits at 723 nm and can be excited with a 633 nm red laser. Instruments typically require a 685 LP mirror and 710/20 filter.

Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFN-γ (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. Human IFN-γ does not cross-react with mouse IFN-γ.

Functions: