Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 700, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
• Brilliant Violet 650
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 4S.B3 monoclonal antibody reacts with human interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells. IFN-γ exhibits antiviral and antiparasitic activity and inhibits proliferation of normal and transformed cells in synergy with other cytokines such as TNF-α.

This antibody is validated for intracellular staining and flow cytometric analysis using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and BestProtocols®: Protocol A for cytoplasmic proteins. Recommended usage is 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Alexa Fluor® 700 emits at 723 nm and can be excited with a 633 nm red laser. Instruments typically require a 685 LP mirror and 710/20 filter.

Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFN-γ (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. Human IFN-γ does not cross-react with mouse IFN-γ.

Functions:

• Antiviral and antiproliferative activity
• Induction of MHC class I and II
• Macrophage activation
• Enhanced immunoglobulin secretion by B cells
• Cytokine regulation via JAK-STAT pathway

Regulation:

• Upregulated by IL-2, FGF basic, EGF
• Downregulated by Vitamin D3 or DMN
• Mutations associated with aplastic anemia

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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