Thermo Fisher Scientific TGF beta-1,2,3 Monoclonal Antibody (1D11.16.8), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

ELISA (ELISA)

-

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

-

Inhibition Assays (IA)

-

View 1 publication 1 publication

T-Cell Activation (TCA)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D11.16.8

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866348

Product Specific Information

Description: The monoclonal antibody 1D11.16.8 recognizes human, mouse, rat, hamster, canine and non-human primate Transforming Growth Factor (TGF) beta 1, 2 and 3. TGF beta is a pleiotropic cytokine that exists as five isoforms (TGF beta 1-5) that share 70-80% homology with each other. However, these isoforms are not homologous to TGF alpha. TGF beta 1 is highly conserved between human, murine, simian, bovine, porcine, and chicken proteins. TGF beta 1 is the most abundant isoform and is ubiquitously expressed by a variety of cell types, including platelets, macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. However, the other isoforms exhibit a more restricted expression pattern.

TGF beta 1 is synthesized as a long precursor polypeptide, which is cleaved to yield the mature protein and the Latency Associated Peptide (LAP). LAP and mature TGF beta 1 remain non-covalently associated during secretion, forming homodimers known as the Small Latent Complex (SLC). Secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. The bioactivity of mature TGF beta 1 is dependent on its release from LAP by conformational changes and proteolytic processing. Its activities include inhibition of cell growth in epithelial cells, endothelial cells, fibroblasts, neurons, lymphoid cells, and other hematopoietic cell types. TGF beta 1 also inhibits the proliferation of T and NK cells, downregulates the activities of activated macrophages, and blocks the anti-tumor activity of IL-2 - bearing lymphokine-activated killer (LAK) cells. Recently, TGF beta 1 has been found to play a critical role in the development of regulatory T cells and act as a costimulatory factor for expression of Foxp3. Dendritic cells exposed to tumors have been reported to secrete TGF beta 1 and stimulate the differntiation of CD4+CD25+ Treg cell from peripheral CD4+CD25- progeny. TGF beta 1-induced regulatory T cells have been termed iTregs.

Applications Reported: This 1D11.16.8 antibody has been reported for use in neutralization assays both in vivo and in vitro and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.