Thermo Fisher Scientific TGF alpha Monoclonal Antibody (TGF88)
TGF-alpha 단백질을 인식하는 마우스 단클론 항체로, IHC 및 ELISA에 적합. 인간 시료 반응성이 있으며, 비결합형 액상 포맷으로 제공. Protein A 정제 및 안정화 PBS 버퍼 포함. 세포막, 세포질, 핵에서 발현되는 TGF-alpha 검출에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| ELISA | 0.01–0.1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TGF88 |
| Immunogen | Recombinant protein of 50 aa TGF-alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Cirrhotic liver
- Cellular location: Membrane, cytoplasm, and nucleus
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells.
TGF-alpha induces anchorage-independent transformation in cultured cells. Human, murine, and rat TGF-alpha are cross-species reactive.
TGF-alpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing a reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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