Thermo Fisher Scientific eIF4AIII/EIF4A3 Polyclonal Antibody
Rabbit polyclonal antibody targeting eIF4AIII/EIF4A3 for WB, IHC, and IP applications. Recognizes conserved region 125–175 of human eIF4A3. Suitable for multiple species. Supplied as liquid, unconjugated form, 0.20 mg/mL, stored at 4°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 125 and 175 of human Eukaryotic Translation Initiation Factor 4A3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted to react with Rat, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, and Pig based on 100% sequence identity
Target Information
eIF4A3 is a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are putative RNA helicases involved in cellular processes that alter RNA secondary structure, including translation initiation, splicing, and ribosome/spliceosome assembly. Some family members are associated with embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein with high sequence similarity to translation initiation factors eIF4AI and eIF4AII.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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