Thermo Fisher Scientific eIF4AIII/EIF4A3 Polyclonal Antibody
Thermo Fisher Scientific의 eIF4AIII/EIF4A3 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 Rabbit IgG 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, RNA 헬리케이스 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 125 and 175 of human Eukaryotic Translation Initiation Factor 4A3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted to react with Rat, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, and Pig (based on 100% sequence identity)
Target Information
eIF4A3 is a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are putative RNA helicases involved in cellular processes such as translation initiation, RNA splicing, and ribosome assembly. eIF4A3 functions as a nuclear matrix protein and shares high sequence similarity with eIF4AI and eIF4AII, other members of the DEAD box family. These proteins are implicated in embryogenesis, spermatogenesis, and cellular growth and division.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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