Thermo Fisher Scientific eIF4AIII/EIF4A3 Polyclonal Antibody
eIF4AIII/EIF4A3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등 다양한 응용에 사용 가능. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 4A3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Rat, Bovine, and Pig
Target Information
eIF4A3 is a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are putative RNA helicases involved in various RNA secondary structure modifications, including translation initiation, splicing, and assembly of ribosomes and spliceosomes. Some members are associated with embryogenesis, spermatogenesis, and cell growth and division. eIF4A3 is a nuclear matrix protein, sharing high sequence similarity with eIF4AI and eIF4AII.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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