Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 488
6x-His 태그 단백질 검출용 DyLight 488 형광 접합 단클론 항체. Western blot 및 면역형광(ICC/IF)에 최적화. 마우스 IgG2b 아이소타입, Protein A 정제. 492/519 nm 형광 특성으로 높은 감도와 특이성 제공.
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Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS.H8 |
| Immunogen | 6x His synthetic peptide |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536984 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 550
- DyLight 650
- DyLight 680
- HRP
- Custom conjugation available upon request
Product Specific Information
MA1-21315-D488 has been successfully used for immunofluorescence.
DyLight 488 has an excitation/emission of 493/518 nm.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly attached to recombinant proteins at the N- or C-terminus to enable purification by immobilized metal affinity chromatography.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
The histidine cluster binds selectively to Ni²⁺ ions, allowing efficient purification using Ni²⁺-NTA adsorbents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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