Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 650
6x-His 태그 단백질 검출용 DyLight 650 형광 표지 단일클론 항체. Western blot, ICC/IF, Flow cytometry에 활용 가능. 높은 특이성과 안정성, 4°C 보관. 연구용으로 적합.
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Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 650
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:25–1:100 | View 2 publications |
| Flow Cytometry (Flow) | - | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS.H8 |
| Immunogen | 6x His synthetic peptide |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536986 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 488
- DyLight 550
- DyLight 680
- HRP
- Request custom conjugation available
Product Specific Information
MA1-21315-D650 has been successfully used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672 nm.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal region of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
The strong and selective affinity of histidine clusters to Ni²⁺ allows efficient purification using Ni²⁺-NTA adsorbents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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