Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8)
6x-His 태그 단백질 검출용 단일클론 항체로 Western blot, ICC, ELISA 등 다양한 응용에 적합. 마우스 IgG2b 형식의 비결합 항체이며, 고순도 Protein A 정제. 단기 4°C, 장기 -20°C 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | 170 |
| Immunohistochemistry (IHC) | – | 5 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 | 10 |
| Flow Cytometry (Flow) | – | 5 |
| ELISA | Assay-dependent | 21 |
| Immunoprecipitation (IP) | Assay-dependent | 19 |
| Neutralization (Neu) | – | 1 |
| Functional Assay | – | 2 |
| Inhibition Assays (IA) | – | 1 |
| In vitro Assay (IV) | – | 2 |
| Miscellaneous (PubMed) | – | 5 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS.H8 |
| Immunogen | 6x His synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Dry ice (international) |
| RRID | AB_557403 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- HRP
- Custom conjugation available upon request
Product Specific Information
MA1-21315 may be stored at 4°C for short-term use. Add 0.05% sodium azide if desired. For long-term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly fused to the N- or C-terminal region of recombinant proteins to facilitate purification by immobilized metal affinity chromatography (IMAC).
His tag antibodies enable detection and immunoprecipitation of tagged recombinant proteins even when specific antibodies for the target protein are unavailable. The histidine cluster binds strongly to metal ions such as Ni²⁺, allowing purification of His-tagged proteins to homogeneity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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