Thermo Fisher Scientific ARL3 Monoclonal Antibody (OTI3D3), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily.
The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARL3 and 43% with human ARF1.
Like ARFs, ARL3 has a glycine at position 2 (N-myristoylation site) and lacks cysteine residues near the C-terminus.
Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung.
A 5.5-kb transcript was also found in most tissues, highest in brain.
Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells.
Although ARL3 binds GTP, it lacks cholera toxin-dependent ADP-ribosylation activity and is classified as an ARF-like protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.