Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737
• Brilliant Violet 421, 480, 650, 786
• eFluor 450
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 702
• Custom conjugation available upon request

Product Specific Information

Description:
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. Ly-6G plays roles in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a and CD11b. The ligand for Ly-6G is unknown.

Unlike the RB6-8C5 antibody, 1A8-Ly6g binds specifically to Ly-6G on neutrophils and has been used to deplete neutrophils with reported antagonist activity.

Applications Reported:

• Flow cytometric analysis
• Depletion of neutrophils
• Functional antagonist studies

Applications Tested:

• Tested by flow cytometric analysis of mouse bone marrow cells
• Recommended ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimize titration for best performance

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtered

Purity and Quality:

• Purity: >90% (SDS-PAGE)
• Endotoxin: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D):
Component of the myeloid differentiation antigen Gr-1, together with Ly6C.

• Molecular weight: 21–25 kDa
• Expressed by myeloid-derived cells in bone marrow
• Marker for neutrophils, monocytes, and granulocytes
• Expression correlates with granulocyte differentiation and maturation
• Involved in anti-tumor response and regulation of local immune responses

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.