Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Functional Grade, eBioscience
마우스 Ly-6G(Gr-1)에 특이적인 단클론 항체로, 호중구 표지 및 제거 연구에 적합합니다. Flow cytometry와 기능적 분석에 사용 가능하며, 0.25 µg/test로 최적화되었습니다. 1 mg/mL 농도의 액상 제품으로 4°C 보관 및 wet ice 배송됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 5 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 3 |
| Flow Cytometry (Flow) | 0.25 µg/test | 35 |
| Functional Assay (Functional) | Assay-Dependent | 1 |
| Inhibition Assays (IA) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse, Virus |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2866366 |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737
- Brilliant Violet 421, 480, 650, 786
- eFluor 450
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 702
- Custom conjugation available upon request
Product Specific Information
Description:
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. Ly-6G plays roles in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a and CD11b. The ligand for Ly-6G is unknown.
Unlike the RB6-8C5 antibody, 1A8-Ly6g binds specifically to Ly-6G on neutrophils and has been used to deplete neutrophils with reported antagonist activity.
Applications Reported:
- Flow cytometric analysis
- Depletion of neutrophils
- Functional antagonist studies
Applications Tested:
- Tested by flow cytometric analysis of mouse bone marrow cells
- Recommended ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimize titration for best performance
Storage and Handling:
- Use in sterile environment
- 0.2 µm post-manufacturing filtered
Purity and Quality:
- Purity: >90% (SDS-PAGE)
- Endotoxin: <0.001 ng/µg antibody (LAL assay)
- Aggregation: <10% (HPLC)
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D):
Component of the myeloid differentiation antigen Gr-1, together with Ly6C.
- Molecular weight: 21–25 kDa
- Expressed by myeloid-derived cells in bone marrow
- Marker for neutrophils, monocytes, and granulocytes
- Expression correlates with granulocyte differentiation and maturation
- Involved in anti-tumor response and regulation of local immune responses
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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