Thermo Fisher Scientific TIGIT Monoclonal Antibody (GIGD7), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 8 publications 8 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GIGD7

Immunogen

mTIGIT-Fc treated with 4% PFA

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 702
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866363

Product Specific Information

Description: The GIGD7 monoclonal antibody reacts with mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT, VSTM3, WUCAM), a 26 kDa protein that is a member of the poliovirus receptor (PVR) family. In mice, the expression of TIGIT has been reported in follicular T helper cells, while in humans, the expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells. TIGIT is not expressed on B cells or naive CD4+ T cells. TIGIT is upregulated on CD4+ T cells following activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK cells and T cells with antigen presenting cells, fibroblasts and endothelial cells that express PVR and PVR-like proteins.

Applications Reported: This GIGD7 antibody has been reported for use in flow cytometric analysis. (Fluorochrome-conjugated GIGD7 is recommended for use in flow cytometry).

Applications Tested: This GIGD7 antibody has been tested by flow cytometric analysis of mouse TIGIT-transfected cell lines. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.